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Giemsa Solution


Product Description

Polychromatic Romanowsky dyes are a standard in hematology of blood smears and bone marrow. Various sorts of Romanowsky dyes (Giemsa, May-Gruenwald, Leishman, Wright, Jenner and others) contain different ratios of methylene blue used as the cation component (and the reagent-related thiazine dyes, such as azure B) and eosin Y as the anion component. Cation and anion components interaction creates a well-known Romanowsky effect that cannot be achieved if each component is being used individually. Purple color indicates the effect’s presence. Staining intensity depends on the azure B content, as well as azure B to eosin Y ratio, while a few other factors affect the result of staining: working solution pH value, fixation method and dye exposure time. BioGnost’s Giemsa solution is used for differentiation of nuclear and/or cytoplasmatic morphology of lymphocytes, monocytes, granulocytes (neutrophils, eosinophils, basophils), thrombocytes and erythrocytes. There are various methods of using the Giemsa solution, and the so-called Pappenheim method is one of the most commonly used ones. The method is essentially the May-Gruenwald Giemsa method combined with the May-Gruenwald solution that stains cytological material (peripheral blood smears, cytodiagnostic puncture aspirates, diarrhea or secretion cells) or hematopoietic organs’ sections. Along with the Pappenheim method, the Giemsa solution is commonly used for chromosomatic aberrations detection in cytogenetics.

Specifications


Cat. No.


Size


Purpose Of Usage


Storage Conditions

GM-OT-100, GM-OT-110, GM-OT-500, GM-OT-1L, GM-OT-2,5L


100mL, 10x100mL, 500mL, 1000mL, 2,5Lt


CE IVD


15-25 °C


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